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Protein concentrations of mit have been based on regimen (electronic.h., bicinchoninic chemical p) assays. Supernatants that contains A single mg/mL regarding proteins each and every were labeled along with One particular mg/mL biotin-PEO4-NHS at 4��C for 20 minutes. Biotinylated cell meats had been television through a 0.2-��m centrifuge filtration (Millipore, Billerica Mum), packed upon the Sup
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International students are topic to the Dutch immigration procedures. تا اين اواخر،سياست هاي مهاجرت استراليا، انگليس ها را به اقامت دائم و تابعيت دراستراليا تشويق مي كرد، به موجب اين سياست امروزه، تقريبا تمام افراد انگليسي حداقل يك خويشاوند در استراليا دارند اما اين قانون مهاجرت تا اندازه اي تغيير كرده است و دولت جديد استراليا تمايلي به پذيرش تقاضا نامه هاي مهاجرت افراد انگليسي نيست، بلكه فقط ب
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